Based on a recessional velocity of 1200 km/sec, NGC 4350 is about 55 million light years away, in good agreement with redshift-independent distance estimates of 50 to 55 million light years. Given that and its apparent size of 3.0 by 1.4 arcmin, it is about 50 thousand light years across. The galaxy is generally thought to be paired with NGC 4340.

Part of Markarian's Chain, a group of Virgo Cluster galaxies which lie along a smooth curve and have similar radial velocities.

A group of Virgo Cluster galaxies (NGC 4374 (M84), NGC 4406 (M86), 4435, 4438, 4458, 4461, 4473 and NGC 4477) noted by Markarian (in the 1960's) as lying on a smooth curved arc, and having similar motions (meaning similar recessional velocities, as proper motions cannot be measured for other galaxies, even with present technology).

A chaotic galaxy with several bright ionized hydrogen (HII) regions, three of which received their own NGC designations: NGC 4399, 4400, and 4401. The recessional velocity of 320 km/sec is too small to be a reliable distance indicator, as the peculiar (non-Hubble expansion) velocity for the galaxy could be a substantial portion of the value. But as it happens, the 15 million light year distance implied by the recessional velocity is in good agreement with redshift-independent distance estimates of 12 to 15 million light years. Given that and its apparent size of 13.2 by 11.0 arcmins, NGC 4395 is about 55 thousand light years across.

Part of NGC 4395. Dreyer lists NGC 4399 as reported by Stoney's employer, William Parsons, but notes that most of his discoveries were actually made by his assistants.